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◦ c m1 inos monoclonal mouse igg mab9502 r d systems minneapolis mn m2 cd206 polyclonal goat igg af2535 r d systems minneapolis mn  (R&D Systems)


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    R&D Systems ◦ c m1 inos monoclonal mouse igg mab9502 r d systems minneapolis mn m2 cd206 polyclonal goat igg af2535 r d systems minneapolis mn
    Fig. 3. Emp2 KO mice have an increased percentage of M1 macrophages in the placenta at GD12.5 compared to WT mice. (A) Graphs depict the percentage of macro phages (F4/80+) cells of CD45+ cells isolated from the decidua, placenta, and uterus in WT (open bars) and Emp2 KO (grey bars) mice. Macrophages represent the largest percentage of immune cells in all placental tissues, and do display differences in the percentage of CD45+ cells across the tissue types in WT (*p = 0.03, ANOVA), but not Emp2 KO mice. However, there are no differences between WT and Emp2 KO mouse macrophage percentages in the uterus, decidua or placenta (n = 4-6 samples/ group). B) Graphs depict the percentage of M1 macrophages (MHCII+) from the total F4/80+ cells isolated from the decidua, placenta, and uterus in WT (open bars) and Emp2 KO (grey bars) mice. WT mice display significant differ ences in the percentage of MHCII + cells across the tissue types (**p = 0.005, ANOVA), with the largest percentage of M1 macrophages present in the decidua. Emp2 KO mice do not display significant differences in the M1 macrophage percentage in the various tissues. However, there is an increased percentage of M1 macrophages in Emp2 KO mice in the placenta compared to WT mice (*p = 0.03, Student’s t test). C) Graphs depict the percent age of M2 macrophages <t>(CD206+)</t> from the F4/ 80+ cells isolated from the decidua, placenta, and uterus in WT (open bars) and Emp2 KO (grey bars) mice (n = 4-5 samples/group). Emp2 KO mice display differences in the per centage of CD206+ macrophages across the tissue types (*p = 0.04, ANOVA), with the largest percentage of M2 macrophages present in the placenta. WT mice do not display sig nificant differences in the M2 macrophage percentage across the various tissues. D) How ever, there is an increased M1/M2 macrophage ratio in Emp2 KO mice in the placenta compared to WT mice (*p = 0.03, Student’s t test). Data are represented as mean ± SEM and show the compiled results from 3 independent experiments using pooled samples from at least 3 mice/group.
    ◦ C M1 Inos Monoclonal Mouse Igg Mab9502 R D Systems Minneapolis Mn M2 Cd206 Polyclonal Goat Igg Af2535 R D Systems Minneapolis Mn, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 78 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+human+cd206+monoclonal+antibody/Human%2FMouse%2FRat+iNOS+Antibody/pm33774530-93-17-24
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    Images

    1) Product Images from "Epithelial membrane protein 2 (Emp2) modulates innate immune cell population recruitment at the maternal-fetal interface."

    Article Title: Epithelial membrane protein 2 (Emp2) modulates innate immune cell population recruitment at the maternal-fetal interface.

    Journal: Journal of reproductive immunology

    doi: 10.1016/j.jri.2021.103309

    Fig. 3. Emp2 KO mice have an increased percentage of M1 macrophages in the placenta at GD12.5 compared to WT mice. (A) Graphs depict the percentage of macro phages (F4/80+) cells of CD45+ cells isolated from the decidua, placenta, and uterus in WT (open bars) and Emp2 KO (grey bars) mice. Macrophages represent the largest percentage of immune cells in all placental tissues, and do display differences in the percentage of CD45+ cells across the tissue types in WT (*p = 0.03, ANOVA), but not Emp2 KO mice. However, there are no differences between WT and Emp2 KO mouse macrophage percentages in the uterus, decidua or placenta (n = 4-6 samples/ group). B) Graphs depict the percentage of M1 macrophages (MHCII+) from the total F4/80+ cells isolated from the decidua, placenta, and uterus in WT (open bars) and Emp2 KO (grey bars) mice. WT mice display significant differ ences in the percentage of MHCII + cells across the tissue types (**p = 0.005, ANOVA), with the largest percentage of M1 macrophages present in the decidua. Emp2 KO mice do not display significant differences in the M1 macrophage percentage in the various tissues. However, there is an increased percentage of M1 macrophages in Emp2 KO mice in the placenta compared to WT mice (*p = 0.03, Student’s t test). C) Graphs depict the percent age of M2 macrophages (CD206+) from the F4/ 80+ cells isolated from the decidua, placenta, and uterus in WT (open bars) and Emp2 KO (grey bars) mice (n = 4-5 samples/group). Emp2 KO mice display differences in the per centage of CD206+ macrophages across the tissue types (*p = 0.04, ANOVA), with the largest percentage of M2 macrophages present in the placenta. WT mice do not display sig nificant differences in the M2 macrophage percentage across the various tissues. D) How ever, there is an increased M1/M2 macrophage ratio in Emp2 KO mice in the placenta compared to WT mice (*p = 0.03, Student’s t test). Data are represented as mean ± SEM and show the compiled results from 3 independent experiments using pooled samples from at least 3 mice/group.
    Figure Legend Snippet: Fig. 3. Emp2 KO mice have an increased percentage of M1 macrophages in the placenta at GD12.5 compared to WT mice. (A) Graphs depict the percentage of macro phages (F4/80+) cells of CD45+ cells isolated from the decidua, placenta, and uterus in WT (open bars) and Emp2 KO (grey bars) mice. Macrophages represent the largest percentage of immune cells in all placental tissues, and do display differences in the percentage of CD45+ cells across the tissue types in WT (*p = 0.03, ANOVA), but not Emp2 KO mice. However, there are no differences between WT and Emp2 KO mouse macrophage percentages in the uterus, decidua or placenta (n = 4-6 samples/ group). B) Graphs depict the percentage of M1 macrophages (MHCII+) from the total F4/80+ cells isolated from the decidua, placenta, and uterus in WT (open bars) and Emp2 KO (grey bars) mice. WT mice display significant differ ences in the percentage of MHCII + cells across the tissue types (**p = 0.005, ANOVA), with the largest percentage of M1 macrophages present in the decidua. Emp2 KO mice do not display significant differences in the M1 macrophage percentage in the various tissues. However, there is an increased percentage of M1 macrophages in Emp2 KO mice in the placenta compared to WT mice (*p = 0.03, Student’s t test). C) Graphs depict the percent age of M2 macrophages (CD206+) from the F4/ 80+ cells isolated from the decidua, placenta, and uterus in WT (open bars) and Emp2 KO (grey bars) mice (n = 4-5 samples/group). Emp2 KO mice display differences in the per centage of CD206+ macrophages across the tissue types (*p = 0.04, ANOVA), with the largest percentage of M2 macrophages present in the placenta. WT mice do not display sig nificant differences in the M2 macrophage percentage across the various tissues. D) How ever, there is an increased M1/M2 macrophage ratio in Emp2 KO mice in the placenta compared to WT mice (*p = 0.03, Student’s t test). Data are represented as mean ± SEM and show the compiled results from 3 independent experiments using pooled samples from at least 3 mice/group.

    Techniques Used: Isolation

    Fig. 5. NK cell depletion in Emp2 KO mice leads to increased macrophage recruitment. A. Representative images taken at 100X magnification of the placenta taken from WT or Emp2 KO mice treated with control non- targeted IgG antibody or anti-asialo GM1 in jections. Sections were stained for M1 macro phages or M2 macrophages using anti-iNOS (Texas Red) or anti-CD206 (FITC) antibodies, respectively, and nuclei were counterstained using DAPI. The division between the junc tional zone (JZ) and decidua was demarcated using the nuclei of trophoblast giant cells. Merged images are shown. Scale bar = 200 μm. B. Semi-quantitative determination of the mean staining intensity of M1 (Texas-Red) and M2 (FITC) macrophages in WT and Emp2 KO mice treated with anti-asialo GM1 vs control IgG in jections. Graph represents the mean pixel count for each color ± SEM from 3 independent ex periments using isolated decidua from at least 3 mice/group. A one way ANOVA was used to compare the means between groups.
    Figure Legend Snippet: Fig. 5. NK cell depletion in Emp2 KO mice leads to increased macrophage recruitment. A. Representative images taken at 100X magnification of the placenta taken from WT or Emp2 KO mice treated with control non- targeted IgG antibody or anti-asialo GM1 in jections. Sections were stained for M1 macro phages or M2 macrophages using anti-iNOS (Texas Red) or anti-CD206 (FITC) antibodies, respectively, and nuclei were counterstained using DAPI. The division between the junc tional zone (JZ) and decidua was demarcated using the nuclei of trophoblast giant cells. Merged images are shown. Scale bar = 200 μm. B. Semi-quantitative determination of the mean staining intensity of M1 (Texas-Red) and M2 (FITC) macrophages in WT and Emp2 KO mice treated with anti-asialo GM1 vs control IgG in jections. Graph represents the mean pixel count for each color ± SEM from 3 independent ex periments using isolated decidua from at least 3 mice/group. A one way ANOVA was used to compare the means between groups.

    Techniques Used: Control, Staining, Isolation

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    Blocking Assay:

    Article Title: Macrophage Mannose Receptor CD206 Predicts Prognosis in Community-acquired Pneumonia
    Article Snippet: .. Deparaffinized sections (5-μm-thick) were immersed in epitope retrieval solution (Target Retrieval Solution S1700; Dako North America, Inc., Carpinteria, CA, USA) and preheated at 120 °C for 10 min. After blocking endogenous peroxidase activity with 3% H 2 O 2 for 15 min, slides were incubated overnight with a mouse anti-human CD206 monoclonal antibody (15 μg/ml; R&D Systems, Minneapolis, MN, USA) or IgG2b at 4 °C. .. Subsequently, sections were incubated with visualization reagent (ChemMate Envision kit; Dako Japan, Inc., Tokyo, Japan) for 30 min, followed by counterstaining with hematoxylin.

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    Article Snippet: .. Deparaffinized sections (5-μm thick) were immersed in epitope retrieval solution (Target Retrieval Solution S1700; Dako North America, Inc., Carpinteria, CA, USA) and preheated at 120 °C for 10 min. After blocking endogenous peroxidase activity with 3% H 2 O 2 for 15 min, slides were incubated overnight with a mouse anti-human CD206 monoclonal antibody (15 μg/ml; R&D Systems, Minneapolis, MN, USA) or IgG2b at 4 °C. .. Subsequently, sections were incubated with visualization reagent (ChemMate Envision kit; Dako Japan, Inc., Tokyo, Japan) for 30 min, followed by counterstaining with haematoxylin.

    Activity Assay:

    Article Title: Macrophage Mannose Receptor CD206 Predicts Prognosis in Community-acquired Pneumonia
    Article Snippet: .. Deparaffinized sections (5-μm-thick) were immersed in epitope retrieval solution (Target Retrieval Solution S1700; Dako North America, Inc., Carpinteria, CA, USA) and preheated at 120 °C for 10 min. After blocking endogenous peroxidase activity with 3% H 2 O 2 for 15 min, slides were incubated overnight with a mouse anti-human CD206 monoclonal antibody (15 μg/ml; R&D Systems, Minneapolis, MN, USA) or IgG2b at 4 °C. .. Subsequently, sections were incubated with visualization reagent (ChemMate Envision kit; Dako Japan, Inc., Tokyo, Japan) for 30 min, followed by counterstaining with hematoxylin.

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    Article Title: Macrophage mannose receptor, CD206, predict prognosis in patients with pulmonary tuberculosis
    Article Snippet: .. Deparaffinized sections (5-μm thick) were immersed in epitope retrieval solution (Target Retrieval Solution S1700; Dako North America, Inc., Carpinteria, CA, USA) and preheated at 120 °C for 10 min. After blocking endogenous peroxidase activity with 3% H 2 O 2 for 15 min, slides were incubated overnight with a mouse anti-human CD206 monoclonal antibody (15 μg/ml; R&D Systems, Minneapolis, MN, USA) or IgG2b at 4 °C. .. Subsequently, sections were incubated with visualization reagent (ChemMate Envision kit; Dako Japan, Inc., Tokyo, Japan) for 30 min, followed by counterstaining with haematoxylin.

    Incubation:

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    Article Title: Successful classification of macrophage-mannose receptor CD206 in severity of anti-MDA5 antibody positive dermatomyositis associated ILD.
    Article Snippet: Objectives.. Macrophage-mannose receptor, CD206, is a marker of alternatively activated macrophages.. Activated macrophages play key roles in DM.

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    Fig. 3. Emp2 KO mice have an increased percentage of M1 macrophages in the placenta at GD12.5 compared to WT mice. (A) Graphs depict the percentage of macro phages (F4/80+) cells of CD45+ cells isolated from the decidua, placenta, and uterus in WT (open bars) and Emp2 KO (grey bars) mice. Macrophages represent the largest percentage of immune cells in all placental tissues, and do display differences in the percentage of CD45+ cells across the tissue types in WT (*p = 0.03, ANOVA), but not Emp2 KO mice. However, there are no differences between WT and Emp2 KO mouse macrophage percentages in the uterus, decidua or placenta (n = 4-6 samples/ group). B) Graphs depict the percentage of M1 macrophages (MHCII+) from the total F4/80+ cells isolated from the decidua, placenta, and uterus in WT (open bars) and Emp2 KO (grey bars) mice. WT mice display significant differ ences in the percentage of MHCII + cells across the tissue types (**p = 0.005, ANOVA), with the largest percentage of M1 macrophages present in the decidua. Emp2 KO mice do not display significant differences in the M1 macrophage percentage in the various tissues. However, there is an increased percentage of M1 macrophages in Emp2 KO mice in the placenta compared to WT mice (*p = 0.03, Student’s t test). C) Graphs depict the percent age of M2 macrophages <t>(CD206+)</t> from the F4/ 80+ cells isolated from the decidua, placenta, and uterus in WT (open bars) and Emp2 KO (grey bars) mice (n = 4-5 samples/group). Emp2 KO mice display differences in the per centage of CD206+ macrophages across the tissue types (*p = 0.04, ANOVA), with the largest percentage of M2 macrophages present in the placenta. WT mice do not display sig nificant differences in the M2 macrophage percentage across the various tissues. D) How ever, there is an increased M1/M2 macrophage ratio in Emp2 KO mice in the placenta compared to WT mice (*p = 0.03, Student’s t test). Data are represented as mean ± SEM and show the compiled results from 3 independent experiments using pooled samples from at least 3 mice/group.
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    FIGURE 1. Macrophage infiltration in calcific and noncalcified aortic valves. A, Representative images of immunohistochemical staining for CD68, CD11c, and <t>CD206</t> in the calcific and noncalcified aortic valves. Scale bar ¼ 100 mm. B-D, Cell counts of infiltrating macrophages. Each point represents the cell count of infiltrating macrophages (/mm2). E-G, M1/M2 macrophage polarization in the calcific and noncalcified aortic valves. Each point represents the ratio of (E) CD11cþ and (F) CD206þ cells to CD68þ cells and (G) CD11cþ cells to CD206þ cells. Bars represent mean standard deviation. *P<.05, **P<.01. Color legend: calcific aortic valves (red), noncalcified aortic valves (green). AV, Aortic valve.
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    Image Search Results


    Histopathological analyses of CD68+ (PANEL 1), CD163+ (PANEL 2), and CD206+ (PANEL 3) cells in footpad lesions of WT and CETP mice at 4 and 12-weeks post-infection with L. (L.) amazonensis . Control, uninfected footpad: WT (A) and CETP (B) . Infected: WT (C, E) and CETP (D, F) in the 4th and 12th week PI analyzed under an optical microscope (100x magnification). Representative images from three independent experiments. Arrows indicate the presence of immunostaining in macrophages. (G) Representative graph of cell density (cells/mm2) with the respective macrophage markers. Results expressed as mean ± SD, n= 4–6 per group, compared by Mann-Whitney test: WT vs. CETP 4th and 12th weeks PI; * p <0.05 and ** p <0.005, *** p <0.001.

    Journal: Frontiers in Immunology

    Article Title: The impact of cholesteryl ester transfer protein on the progression of cutaneous leishmaniasis

    doi: 10.3389/fimmu.2024.1389551

    Figure Lengend Snippet: Histopathological analyses of CD68+ (PANEL 1), CD163+ (PANEL 2), and CD206+ (PANEL 3) cells in footpad lesions of WT and CETP mice at 4 and 12-weeks post-infection with L. (L.) amazonensis . Control, uninfected footpad: WT (A) and CETP (B) . Infected: WT (C, E) and CETP (D, F) in the 4th and 12th week PI analyzed under an optical microscope (100x magnification). Representative images from three independent experiments. Arrows indicate the presence of immunostaining in macrophages. (G) Representative graph of cell density (cells/mm2) with the respective macrophage markers. Results expressed as mean ± SD, n= 4–6 per group, compared by Mann-Whitney test: WT vs. CETP 4th and 12th weeks PI; * p <0.05 and ** p <0.005, *** p <0.001.

    Article Snippet: CD206 Mouse Monoclonal , 1/1000 , BioRad/MCA55527.

    Techniques: Infection, Control, Microscopy, Immunostaining, MANN-WHITNEY

    Fig. 3. Emp2 KO mice have an increased percentage of M1 macrophages in the placenta at GD12.5 compared to WT mice. (A) Graphs depict the percentage of macro phages (F4/80+) cells of CD45+ cells isolated from the decidua, placenta, and uterus in WT (open bars) and Emp2 KO (grey bars) mice. Macrophages represent the largest percentage of immune cells in all placental tissues, and do display differences in the percentage of CD45+ cells across the tissue types in WT (*p = 0.03, ANOVA), but not Emp2 KO mice. However, there are no differences between WT and Emp2 KO mouse macrophage percentages in the uterus, decidua or placenta (n = 4-6 samples/ group). B) Graphs depict the percentage of M1 macrophages (MHCII+) from the total F4/80+ cells isolated from the decidua, placenta, and uterus in WT (open bars) and Emp2 KO (grey bars) mice. WT mice display significant differ ences in the percentage of MHCII + cells across the tissue types (**p = 0.005, ANOVA), with the largest percentage of M1 macrophages present in the decidua. Emp2 KO mice do not display significant differences in the M1 macrophage percentage in the various tissues. However, there is an increased percentage of M1 macrophages in Emp2 KO mice in the placenta compared to WT mice (*p = 0.03, Student’s t test). C) Graphs depict the percent age of M2 macrophages (CD206+) from the F4/ 80+ cells isolated from the decidua, placenta, and uterus in WT (open bars) and Emp2 KO (grey bars) mice (n = 4-5 samples/group). Emp2 KO mice display differences in the per centage of CD206+ macrophages across the tissue types (*p = 0.04, ANOVA), with the largest percentage of M2 macrophages present in the placenta. WT mice do not display sig nificant differences in the M2 macrophage percentage across the various tissues. D) How ever, there is an increased M1/M2 macrophage ratio in Emp2 KO mice in the placenta compared to WT mice (*p = 0.03, Student’s t test). Data are represented as mean ± SEM and show the compiled results from 3 independent experiments using pooled samples from at least 3 mice/group.

    Journal: Journal of reproductive immunology

    Article Title: Epithelial membrane protein 2 (Emp2) modulates innate immune cell population recruitment at the maternal-fetal interface.

    doi: 10.1016/j.jri.2021.103309

    Figure Lengend Snippet: Fig. 3. Emp2 KO mice have an increased percentage of M1 macrophages in the placenta at GD12.5 compared to WT mice. (A) Graphs depict the percentage of macro phages (F4/80+) cells of CD45+ cells isolated from the decidua, placenta, and uterus in WT (open bars) and Emp2 KO (grey bars) mice. Macrophages represent the largest percentage of immune cells in all placental tissues, and do display differences in the percentage of CD45+ cells across the tissue types in WT (*p = 0.03, ANOVA), but not Emp2 KO mice. However, there are no differences between WT and Emp2 KO mouse macrophage percentages in the uterus, decidua or placenta (n = 4-6 samples/ group). B) Graphs depict the percentage of M1 macrophages (MHCII+) from the total F4/80+ cells isolated from the decidua, placenta, and uterus in WT (open bars) and Emp2 KO (grey bars) mice. WT mice display significant differ ences in the percentage of MHCII + cells across the tissue types (**p = 0.005, ANOVA), with the largest percentage of M1 macrophages present in the decidua. Emp2 KO mice do not display significant differences in the M1 macrophage percentage in the various tissues. However, there is an increased percentage of M1 macrophages in Emp2 KO mice in the placenta compared to WT mice (*p = 0.03, Student’s t test). C) Graphs depict the percent age of M2 macrophages (CD206+) from the F4/ 80+ cells isolated from the decidua, placenta, and uterus in WT (open bars) and Emp2 KO (grey bars) mice (n = 4-5 samples/group). Emp2 KO mice display differences in the per centage of CD206+ macrophages across the tissue types (*p = 0.04, ANOVA), with the largest percentage of M2 macrophages present in the placenta. WT mice do not display sig nificant differences in the M2 macrophage percentage across the various tissues. D) How ever, there is an increased M1/M2 macrophage ratio in Emp2 KO mice in the placenta compared to WT mice (*p = 0.03, Student’s t test). Data are represented as mean ± SEM and show the compiled results from 3 independent experiments using pooled samples from at least 3 mice/group.

    Article Snippet: Then the primary antibodies at 1:100 dilution in PBS were applied onto each section overnight at 4 ◦C (M1: iNOS, monoclonal mouse IgG, #MAB9502, R&D Systems, Minneapolis, MN; M2: CD206, polyclonal goat IgG, #AF2535, R&D Systems, Minneapolis, MN).

    Techniques: Isolation

    Fig. 5. NK cell depletion in Emp2 KO mice leads to increased macrophage recruitment. A. Representative images taken at 100X magnification of the placenta taken from WT or Emp2 KO mice treated with control non- targeted IgG antibody or anti-asialo GM1 in jections. Sections were stained for M1 macro phages or M2 macrophages using anti-iNOS (Texas Red) or anti-CD206 (FITC) antibodies, respectively, and nuclei were counterstained using DAPI. The division between the junc tional zone (JZ) and decidua was demarcated using the nuclei of trophoblast giant cells. Merged images are shown. Scale bar = 200 μm. B. Semi-quantitative determination of the mean staining intensity of M1 (Texas-Red) and M2 (FITC) macrophages in WT and Emp2 KO mice treated with anti-asialo GM1 vs control IgG in jections. Graph represents the mean pixel count for each color ± SEM from 3 independent ex periments using isolated decidua from at least 3 mice/group. A one way ANOVA was used to compare the means between groups.

    Journal: Journal of reproductive immunology

    Article Title: Epithelial membrane protein 2 (Emp2) modulates innate immune cell population recruitment at the maternal-fetal interface.

    doi: 10.1016/j.jri.2021.103309

    Figure Lengend Snippet: Fig. 5. NK cell depletion in Emp2 KO mice leads to increased macrophage recruitment. A. Representative images taken at 100X magnification of the placenta taken from WT or Emp2 KO mice treated with control non- targeted IgG antibody or anti-asialo GM1 in jections. Sections were stained for M1 macro phages or M2 macrophages using anti-iNOS (Texas Red) or anti-CD206 (FITC) antibodies, respectively, and nuclei were counterstained using DAPI. The division between the junc tional zone (JZ) and decidua was demarcated using the nuclei of trophoblast giant cells. Merged images are shown. Scale bar = 200 μm. B. Semi-quantitative determination of the mean staining intensity of M1 (Texas-Red) and M2 (FITC) macrophages in WT and Emp2 KO mice treated with anti-asialo GM1 vs control IgG in jections. Graph represents the mean pixel count for each color ± SEM from 3 independent ex periments using isolated decidua from at least 3 mice/group. A one way ANOVA was used to compare the means between groups.

    Article Snippet: Then the primary antibodies at 1:100 dilution in PBS were applied onto each section overnight at 4 ◦C (M1: iNOS, monoclonal mouse IgG, #MAB9502, R&D Systems, Minneapolis, MN; M2: CD206, polyclonal goat IgG, #AF2535, R&D Systems, Minneapolis, MN).

    Techniques: Control, Staining, Isolation

    FIGURE 1. Macrophage infiltration in calcific and noncalcified aortic valves. A, Representative images of immunohistochemical staining for CD68, CD11c, and CD206 in the calcific and noncalcified aortic valves. Scale bar ¼ 100 mm. B-D, Cell counts of infiltrating macrophages. Each point represents the cell count of infiltrating macrophages (/mm2). E-G, M1/M2 macrophage polarization in the calcific and noncalcified aortic valves. Each point represents the ratio of (E) CD11cþ and (F) CD206þ cells to CD68þ cells and (G) CD11cþ cells to CD206þ cells. Bars represent mean standard deviation. *P<.05, **P<.01. Color legend: calcific aortic valves (red), noncalcified aortic valves (green). AV, Aortic valve.

    Journal: The Journal of thoracic and cardiovascular surgery

    Article Title: The shift of macrophages toward M1 phenotype promotes aortic valvular calcification.

    doi: 10.1016/j.jtcvs.2017.01.052

    Figure Lengend Snippet: FIGURE 1. Macrophage infiltration in calcific and noncalcified aortic valves. A, Representative images of immunohistochemical staining for CD68, CD11c, and CD206 in the calcific and noncalcified aortic valves. Scale bar ¼ 100 mm. B-D, Cell counts of infiltrating macrophages. Each point represents the cell count of infiltrating macrophages (/mm2). E-G, M1/M2 macrophage polarization in the calcific and noncalcified aortic valves. Each point represents the ratio of (E) CD11cþ and (F) CD206þ cells to CD68þ cells and (G) CD11cþ cells to CD206þ cells. Bars represent mean standard deviation. *P<.05, **P<.01. Color legend: calcific aortic valves (red), noncalcified aortic valves (green). AV, Aortic valve.

    Article Snippet: Immunohistochemical staining was performed as previously described.16 After antigen retrieval, deparaffinized sections of each valve were incubated overnight with mouse monoclonal antihuman CD68 (1:100, Abcam, Cambridge, UK), mouse monoclonal anti-human CD11c (1:100, Abcam), and mouse monoclonal anti-human CD206 (1:200, Abcam) followed by application of a secondary antibody (biotinylated anti-mouse immunoglobulin-G).

    Techniques: Immunohistochemical staining, Staining, Cell Counting, Standard Deviation